Reagents/Equipment/Apparatus:
1. Provided 10 ml glass vial.
2. Any suitable spectrophotometer.
3. Relevant Cuvette or cell of
photometer.
4. Aquagenix Ortho-Phosphate reagent 1
one bottle (50 ml).
5. Aquagenix Ortho-Phosphate reagent 2
one bottle (50 ml).
Sample Collection and Preparation.
1. Make sure to immediately analyze the
sample after collection of sample.
2. Ensure to collect sample in a
suitable and designated bottle for a specific sample.
3. Ensure to conduct this test at room
temperature.
Testing Procedure:
1. Take 10 ml De-ionized water into
cuvette for Blank.
2. Take 10ml sample in which you want to
check phosphate and add 10-12 drops (1 ml approx.) of Ortho-Phosphate reagent 1
and mix well.
3. Add 2-4 drops of Ortho-Phosphate
reagent 2 and mix well.
4. Wait for 10 minutes blue complex of
Phospho-molybdate will appear.
5. Set your spectrophotometer on 880 nm
wavelength.
6. First set zero with blank.
7. Now take sample in cuvette and press
read.
8. Reading appears in mg/l.